软件包：rsem（1.3.3+dfsg-2 以及其他的）
RNA-Seq by Expectation-Maximization
RSEM is a software package for estimating gene and isoform expression levels from RNA-Seq data. The RSEM package provides an user-friendly interface, supports threads for parallel computation of the EM algorithm, single-end and paired-end read data, quality scores, variable-length reads and RSPD estimation. In addition, it provides posterior mean and 95% credibility interval estimates for expression levels. For visualization, It can generate BAM and Wiggle files in both transcript-coordinate and genomic-coordinate. Genomic-coordinate files can be visualized by both UCSC Genome browser and Broad Institute’s Integrative Genomics Viewer (IGV). Transcript-coordinate files can be visualized by IGV. RSEM also has its own scripts to generate transcript read depth plots in pdf format. The unique feature of RSEM is, the read depth plots can be stacked, with read depth contributed to unique reads shown in black and contributed to multi-reads shown in red. In addition, models learned from data can also be visualized. Last but not least, RSEM contains a simulator.
其他与 rsem 有关的软件包
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- dep: libc6 (>= 2.34)
- GNU C 语言运行库：共享库
同时作为一个虚包由这些包填实： libc6-udeb
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- dep: libgcc-s1 (>= 3.0)
- GCC 支持库
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- dep: libhts3 (>= 1.10)
- C library for high-throughput sequencing data formats
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- dep: libstdc++6 (>= 11)
- GNU 标准 C++ 库，第3版
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- dep: perl
- 拉里 沃尔的实用报表提取语言(Perl)
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- dep: python3
- interactive high-level object-oriented language (default python3 version)
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- dep: r-base-core
- GNU R core of statistical computation and graphics system
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- sug: hisat2
- graph-based alignment of short nucleotide reads to many genomes
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- sug: r-bioc-ebseq
- R package for RNA-Seq Differential Expression Analysis