软件包：r-bioc-hsmmsinglecell（1.26.0-1）

[ 源代码： r-bioc-hsmmsinglecell  ]

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Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts

Skeletal myoblasts undergo a well-characterized sequence of morphological and transcriptional changes during differentiation. In this experiment, primary human skeletal muscle myoblasts (HSMM) were expanded under high mitogen conditions (GM) and then differentiated by switching to low-mitogen media (DM). RNA-Seq libraries were sequenced from each of several hundred cells taken over a time-course of serum- induced differentiation. Between 49 and 77 cells were captured at each of four time points (0, 24, 48, 72 hours) following serum switch using the Fluidigm C1 microfluidic system. RNA from each cell was isolated and used to construct mRNA-Seq libraries, which were then sequenced to a depth of ~4 million reads per library, resulting in a complete gene expression profile for each cell.

其他与 r-bioc-hsmmsinglecell 有关的软件包

• dep: r-api-4.0

  软件包暂时不可用

• dep: r-api-bioc-3.20

  本虚包由这些包填实： r-bioc-biocgenerics

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