Пакунок: r-bioc-hsmmsinglecell (1.26.0-1)

[ Source: r-bioc-hsmmsinglecell  ]

Експериментальний пакунок

Warning: This package is from the experimental distribution. That means it is likely unstable or buggy, and it may even cause data loss. Please be sure to consult the changelog and other possible documentation before using it.

Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts

Skeletal myoblasts undergo a well-characterized sequence of morphological and transcriptional changes during differentiation. In this experiment, primary human skeletal muscle myoblasts (HSMM) were expanded under high mitogen conditions (GM) and then differentiated by switching to low-mitogen media (DM). RNA-Seq libraries were sequenced from each of several hundred cells taken over a time-course of serum- induced differentiation. Between 49 and 77 cells were captured at each of four time points (0, 24, 48, 72 hours) following serum switch using the Fluidigm C1 microfluidic system. RNA from each cell was isolated and used to construct mRNA-Seq libraries, which were then sequenced to a depth of ~4 million reads per library, resulting in a complete gene expression profile for each cell.

Інші пакунки пов'язані з r-bioc-hsmmsinglecell

• dep: r-api-4.0

  Пакунок недоступний

• dep: r-api-bioc-3.20

  virtual package provided by r-bioc-biocgenerics

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