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[ Source: seqtools  ]

Paketti: dotter (4.44.1+dfsg-3)

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detailed comparison of two genomic sequences

Dotter is a graphical dot-matrix program for detailed comparison of two sequences.

 * Every residue in one sequence is compared to every residue in the
   other, and a matrix of scores is calculated.
 * One sequence is plotted on the x-axis and the other on the y-axis.
 * Noise is filtered out so that alignments appear as diagonal lines.
 * Pairwise scores are averaged over a sliding window to make the score
   matrix more intelligible.
 * The averaged score matrix forms a three-dimensional landscape,
   with the two sequences in two dimensions and the height of the
   peaks in the third. This landscape is projected onto two
   dimensions using a grey-scale image - the darker grey of a peak,
   the higher the score is.
 * The contrast and threshold of the grey-scale image can be adjusted
   interactively, without having to recalculate the score matrix.
 * An Alignment Tool is provided to examine the sequence alignment that
   the grey-scale image represents.
 * Known high-scoring pairs can be loaded from a GFF file and overlaid
   onto the plot.
 * Gene models can be loaded from GFF and displayed alongside the
   relevant axis.
 * Compare a sequence against itself to find internal repeats.
 * Find overlaps between multiple sequences by making a dot-plot of all
   sequences versus themselves.
 * Run Dotter in batch mode to create large, time-consuming dot-plots as
   a background process.

Tagit: Interface Toolkit: GTK

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