C++ htslib/bwa-mem/fermi interface for interrogating sequence data
C++ API and command line tool that provides a rapid and user-friendly
interface to BAM/SAM/CRAM files, global sequence alignment operations
and sequence assembly. Four C libraries perform core operations in
SeqLib: HTSlib for BAM access, BWA-MEM and BLAT for sequence alignment
and Fermi for error correction and sequence assembly. Benchmarking
indicates that SeqLib has lower CPU and memory requirements than leading
C++ sequence analysis APIs. Minimal SeqLib code can extract, error-correct
and assemble reads from a CRAM file and then align with BWA-MEM.
SeqLib also provides additional capabilities, including chromosome-aware
interval queries and read plotting. Command line tools are available for
performing integrated error correction, micro-assemblies and alignment.
